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KMID : 0854720090290040249
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Korean Journal of Asthma, Allergy and Clinical Immunology
2009 Volume.29 No. 4 p.249 ~ p.255
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Analysis of Airway Epithelial Cell Autoantigens Recognized by IgG Autoantibodies from Patients with Severe Asthma and Chronic Obstructive Pulmonary Disease
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Lee Hye-Ah
Park Joo-Hun
Kwon Byul
Choi Gil-Soon
Ye Young-Min
Park Hae-Sim
Nahm Dong-Ho
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Abstract
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Background: The presence of circulating autoantibodies to airway epithelial cells has been reported in patients with asthma and chronic obstructive pulmonary disease (COPD).
Objective: To evaluate the possibility of a common autoantigen associated with both asthma and COPD, we analyzed airway epithelial cell autoantigens recognized by circulating IgG autoantibodies from patients with severe asthma and COPD.
Method: IgG autoantibodies to human airway epithelial cells (A549) were examined in serum samples from patients with severe asthma and COPD by immunoblot analysis.
Result: A 54-kDa airway epithelial autoantigen was commonly recognized by circulating IgG antibodies from patients with severe asthma and COPD. The 54-kDa auto-antigen was identified as cytokeratin 8 protein by mass spectrometry. IgG autoantibodies to recombinant human cytokeratin 8 protein were detected in serum samples from 5 (31.3%) of the 16 patients with severe asthma, 4 (26.6%) of 15 patients with COPD, 1 (7.7%) of 13 patients with mild-to-moderate asthma, and 1 (7.7%) of 13 healthy controls (P=0.056).
Conclusion: In this study, we identified cytokeratin 8 as an airway epithelial autoantigen associated with both severe asthma and COPD. Further studies are needed to determine the role of this autoantigen in the pathogenesis of severe asthma and COPD.
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KEYWORD
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Asthma, Chronic obstructive pulmonary disease, Autoantibody, Autoantigen
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